Ctll2 cell
WebOct 30, 2024 · CTLL2 and Moe7 cells were labeled with tritiated thymidine and treated with different concentrations of IL-15 or Apo-IL15-Sushi. Nuclear incorporation of tritiated thymidine was measured at 48 h. (B) STAT-5 phosphorylation in CD8 + and NK cells as induced by IL-15 and Apo-IL15-Sushi. pSTAT-5 was stained and its expression studied … WebCell Line Description . The CellSensor® irf1-bla CTLL-2 cell line contains a beta-lactamase reporter gene under control of the interferon regulatory factor-1 (irf1) response element …
Ctll2 cell
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WebThe CTLL2 cell line was procured from ATCC, whilst the SKRC52 human renal cell carcinoma cell line was kindly provided by Professor E Oosterwijk from Radboud University Nijmegen Medical Center in The Netherlands. The SKRC52-hFAP cells were prepared following a previously described method . All the cell lines were maintained in an RPMI … WebIL-2/IL-2R signaling is required for T-cell proliferation and other fundamental functions that are essential for the immune response. IL-2 stimulates growth and differentiation of B-cells, NK cells, lymphokine-activated killer cells, monocytes, macrophages and oligodendrocytes. Recombinant Rat IL-2 is a 15.3 kDa protein containing 134 amino ...
Webctll-2 Cells Products Every step of the way, a wide range of cell health products Maintaining healthy cells is the key to experimental success and reproducible research results. WebThe cells require IL-2 for growth and are used to assay for its presence in conditioned media. Application. CTLL-2 has been used in CTLL-2 cell proliferation assay to determine the presence of T-cell cytokines. Culture Medium. RPMI 1640 + 2mM Glutamine + 10-20 IU/ml Human Recombinant IL-2 + 10% Foetal Bovine Serum (FBS).
Web质量控制 本产品通过无菌、支原体等检测。 详情见《产品检测报告》 声明：本细胞产品仅供科学研究之用，严禁转售、共享、分发、出借或赠送给任何第三方。 WebNov 1, 2009 · CTLL2 is a transformed murine cell line and its use may not reflect the activity of soluble IL-15 on lymphocytes in the blood of humans or other primate species. We therefore generated separate IL-15 dependent assays that measured the up-regulation of CD69 on the surface of CD3 − CD8 + lymphocytes in human and cynomolgus blood.
WebJul 22, 2024 · Thanks to cell proliferation assays, which use CTLL2 cells, the activities of these proteins have been validated. Human IL-2 ( 11848-H08H ) Cell proliferation assay …
http://immunservice.com/wp-content/uploads/User-Manual-WST-1-CTLL-2-WST1.CTLL150813-EN-V4.pdf storytime themes for novemberWebCTLL is a subclone of T cells derived from a C57bl/6 mouse. The cells require IL-2 for growth and are used to assay for its presence in conditioned media. Application CTLL-2 … rotary 8546WebAbstract The mouse T-cell line CTLL-2 is known to be dependent on interleukin-2 (IL-2) for both growth and viability. These cells possess high affinity IL-2 receptors and have been … rotary 813WebThermo Fisher Scientific is dedicated to improving the human condition through systems, consumables, and services for researchers. storytime through my window 1993WebActually i am agrwoing ctll2 cells and they are totally grwoing well with good cell viability. and for their culture you should do the following; To make the complete growth medium, … story time themes libraryWebMar 9, 2001 · Man (5)GlcNAc (2)Asn added to CTLL-2 cell cultures inhibited not only phosphorylation of tyrosine kinases but also IL-2-dependent cell proliferation. We found … storytime through my window 1993 bbcWebUsing limiting dilution analysis the HTLp frequency is determined by measuring IL-2 using the IL-2-dependent murine cell line CTLL-2. An important factor associated with the successful performance of any bioassay is the reliability of the indicator cell line used. storytime toys hansel and gretel