WebGateway cloning technology is especially noted for its utility in protein expression. The flexibility and diverse selection of Destination vectors and host systems is particularly attractive for multidisciplinary protein expression studies. ... • pDEST 14, 15, 17, and 24 • pET160 and pET161 DEST vectors: Yeast: pYES2-DEST52: Insect cells ... WebTOPO TA Cloning—To create a Gateway entry clone. Step 1—Produce PCR product. Produce PCR products using Taq polymerase and your own protocol. End the PCR reaction with a final 7 to 30 minute extension step. Step 2—Perform the TOPO Cloning Reaction. Set up one of the following Invitrogen TOPO Cloning reactions using the reagents in the ...

pDEST R4-R3 Sequence and Map - SnapGene

WebMar 2, 2013 · Gateway Cloning Technology is a novel universal system for cloning and subcloning DNA. segments, facilitating gene functional analysis, protein expression, and … WebInvitrogen™ Gateway™ pDEST™17 Vector. Destination vectors for expression in E. coli, insect, yeast, or mammalian cells, as well as for production of native protein or N- or C … cool pictures with keyboard keys

pDEST14 Sequence and Map - SnapGene

WebGateway® pDEST™26 Vector 11809-019 Gateway® pDEST™27 Vector 11812-013 The pcDNA™3.2/V5-DEST Vector has been renamed from pcDNA™3.2-DEST to be more … WebSave to listAdd to cart. The pLenti6⁄V5-DEST™ Gateway™ Vector is a Gateway™-adapted ViraPower™ lentiviral expression vector for lentiviral-based expression of a target gene in dividing and non-dividing mammalian cells. The vector has the CMV promoter for driving constitutive expression of the target gene and the blasticidin selection ... WebpDEST-CMV-3xFLAG-gateway-EGFP was a gift from Robin Ketteler (Addgene plasmid # 122845 ; http://n2t.net/addgene:122845 ; RRID:Addgene_122845) For your References section: Redundancy of human ATG4 protease isoforms in autophagy and LC3/GABARAP processing revealed in cells . cool pictures to write about