WebJul 27, 2024 · The ligase fusion protein may comprise one or more modifications, wherein the ligase, the Halo tag and the additional polypeptide (when applicable) are independently modified through, for example, substitution, deletion, addition, insertion of one or more amino acids, or introduction of moieties or active groups at one or more suitable residues ... WebAbstract. Appending fusion proteins such as affinity tags and fluorescent proteins is important for the study of protein structure and function. The HaloTag® Technology …

Protein Purification Guide An Introduction to Protein Purification ...

WebJun 20, 2008 · The protein tag (HaloTag) is a modified haloalkane dehalogenase designed to covalently bind to synthet … We have designed a modular protein tagging system … WebThe HaloPROTAC ® utilizes the HaloTag protein, an engineered bacterial dehalogenase that allows covalent conjugation of a chloroalkane-labeled molecule to a fusion protein of interest. HaloPROTAC ® s have been designed to induce degradation of various HaloTag fusion substrates, including cytosolic, endosomal and nuclear-localized proteins. lse cutting edge lectures

Expression of Fusion Proteins - Promega Corporation

Fluorophores-CA were either prepared according to literature procedures by B. Matthes or D. Schmidt (MPI-MR), kindly provided by L. Lavis (Janelia Research Campus), or purchased from commercial vendors (Supplementary Table 4). The synthesis of Cyanine3-CA, Cyanine5-CA, and Alexa647-CA is … See more A pET51b(+) vector (Novagen) was used for protein production in E. coli. Proteins were N-terminally tagged with Hisx10, followed by a tobacco etch virus (TEV) protease cleavage … See more We performed crystallization at 20 °C using the vapor-diffusion method. HaloTag9, HaloTag10, and HaloTag11 were labeled with a … See more Proteins were expressed in the E. coli strain BL21(DE3)-pLysS. LB cultures were grown at 37 °C to an optical density at 600 nm (OD600nm) of 0.8, induced by the addition of 0.5 mm … See more Single crystal X-ray diffraction data were collected at 100 K on the X10SA beamline at the SLS (PSI). Data were processed with XDS33. The … See more WebApr 5, 2024 · Firstly, the HaloTag-cutinase fusion protein (HC) with the molecular weight (M w) of 59 kDa was constructed and purified by the recombinant protein technology mentioned above. Moreover, a bifunctional linker was synthesized, whereas the linker molecule has two functional ending group of hexylchloride and p-nitrophenyl phosponate … WebColabeling of HaloTag®-p65 fusion protein with HaloTag® TMR ligand and the Anti-HaloTag® pAb. Cells were labeled with 5µM HaloTag® TMR Ligand for 15 minutes, gently washed and then incubated with 2µg/ml AlexaFluor® 488-conjugated anti-rabbit IgG (Invitrogen). Finally, cells were counterstained with DAPI (Vector Laboratories). l security account