2024 Hygromycin phosphotransferase hpt gene

Hygromycin phosphotransferase hpt gene

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August undefined, 2024

WebHygromycin phosphotransferase (hpt gene): The antibiotic hygromycin is more toxic than neomycin and therefore can kill non-transformed plant cells much faster. … Web1. Digest the pCAMBIA 1301 by SphI (Fig. 5 D) and amplify the hygromycin resistance gene hptII (2146 bp, together with the promoter pCaMV35S, shown in Fig. 5 E) used as …

Hygromycin Resistance Gene Cassettes for Vector Construction and

Web28 sep. 2015 · The HPT gene, encoding hygromycin phosphotransferase and conferring resistance to Hyg (Gritz and Davies 1983), is one of the most widely used selection … WebImmunogen: ABI30072.1 Synonyms: HPT Background: The hygromycin phosphotransferase (denoted hpt, hph or aphIV) gene was originally derived from … how to make skin color clay

Agrobacterium mediated transformation in tomato for disease …

WebThe hygromycin phosphotransferase gene (hpt) from E. coli under the control of the SV40 early promoter was used as a dominant selectable marker for transformation of … Web1 feb. 2009 · Conclusion HPT protein does not have any safety problems to human health. Key words: Hygromycin B phosphotransferase; Selectable marker; Acute toxicity; … Web21 jan. 2024 · The CRISPR/Cas9 technique for rice genome engineering is gaining momentum and requires a precise gene delivery system. For rice and other crop plants, … mt snow ice skating

Prokaryotic Expression and Allergenicity Assessment of …

Agrobacterium tumefaciens-Mediated Transformation of Rice by …

WebThis plasmid contains the hph gene. Catalytic activity ATP + hygromycin B = 4-O-phosphohygromycin B + ADP + H + 1 publication. EC:2.7.1.163 (UniProtKB ENZYME … Webselect transformants expressing the APT gene, which encodes an aminoglycoside phosphotransferase. Hygromycin B (HygB, Wako Pure Chemical Industries, Osaka, Japan) was added to YPD to a ﬁnal concentration of 600mg/ml to select for transformants carrying the HPT gene, encoding HygB phosphotransferase. how to make skin look younger naturallyWebNIKSON HALOMOAN. Segregation Analysis of hpt (hygromycin phosphotransferase) Gene in T0 Generation Transgenic Tobacco Plants. Supervised by SUHARSONO and UTUT … how to make skin glow in photoshop

"WebHygromycin B phosphotransferase (HPT) is a widely used selectable marker protein of transgenic plant. Detection of its activity is critical to studies on the development of … " - Hygromycin phosphotransferase hpt gene

Hygromycin phosphotransferase hpt gene

[Methods of hygromycin B phosphotransferase activity assay in

Webcultivated with Agrobacterium strain EHA101 containing a binary vector pTCL5, having hygromycin phosphotransferase (HPT) gene which confers resistance to hygromycin … WebGen hpt difusikan dengan gen MmCu-Zn-SOD, maka tanaman T0 juga mengandung gen MmCu-Zn-SOD. Kata kunci : segregasi, higromisin, analisis khi-kuadrat. ABSTRACT . …

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Web这一篇我们要为大家讲解的就是抗性基因（resistant gene）在质粒图谱中的AmpR、KanR等表示质粒载体中的筛选标签，多为抗生素抗性基因，方便后续通过抗生素筛选阳 …

Web1 dec. 1991 · Abstract Abstract A general method of gene transfer that does not leave behind a selectable marker in the host genome is described. A luciferase gene was … Web24 sep. 2011 · Of three major selectable genes, the bialaphos resistance (bar) gene and the hygromycin phosphotransferase (hpt) allowed effective selection of transformed cells …

Web1) hygromycin phosphotransferase (hpt) 潮霉素磷酸转移酶基因 (hpt) 2) hygB 潮霉素磷酸转移酶基因 1. We transformed the nematode-trapping fungus Arthrobotrys vermicola … Web25 sep. 2015 · To date, however, only the hygromycin phosphotransferase gene (hpt) has been available for use as a marker for Agrobacterium-mediated transformation in M. …

Web2 sep. 2024 · The hygromycin phosphotransferase (HPT) gene as a selective marker is normally used in screening tests as a first step in detecting and quantifying genetically …

Web1 aug. 2004 · Hygromycin B phosphotransferase (HPT) is a widely used selectable marker protein of transgenic plant. Detection of its activity is critical to studies on the … how to make skin color icingWebTransformation and inheritance of a hygromycin phosphotransferase gene in maize plants. Embryogenic maize (Zea mays L.) callus cultures were transformed by … mt snow grand summit lodgeWebGene: aph4 (hpt) Gene Source: Escherichia coli Product: hygromycin-B phosphotransferase (hph) enzyme Function: allows selection for resistance to the … how to make skinny glass minecraftWebThe aim of this work is to evaluate the use of the hygromycin phosphotransferase (hpt) gene as the selectable marker for generation of stable oil palm transformation via … mt snow in the summerWeb7 aug. 2007 · ABSTRACT: The hygromycin B phosphotransferase gene (hpt) has been widely used in the process of plant genetic engineering to produce plants that can … how to make skin numbWeb5 feb. 2004 · The E. coli gene aphIV (hph, hpt), coding for hygromycin B phosphotransferase (HPT, E.C. 2.7.1.119), confers resistance on bacteria, fungi, … mt snow golf house rentalsWeb27 mei 2024 · During screening of an Arabidopsis population transformed with a hairpin RNA gene-silencing library, we identified some transgenic plants that had active … how to make skin pale in photoshop