WebWe purified the lexA gene product from bacterial strains that bear plasmids that direct the synthesis of large amounts of the protein. Purified lexA protein bound to two … Web26. okt 2024. · LexA is a repressor of a wide variety of SOS genes responsible for a precise and synchronized response that depends on the amount of damage and time since the damage was detected. Self-cleavage...
Mechanism of specific LexA cleavage: Autodigestion and
Web01. nov 2006. · The extremely radioresistant bacterium Deinococcus radiodurans contains two LexA homologues (LexA1 and LexA2) that are possible transcriptional regulators associated with the DNA damage response. In this study, resequencing revealed that there was an additional cytosine nucleotide (nucleotide position 612) in the D. radiodurans … Web17. feb 1986. · The fluorescence of the LexA protein, the common repressor of the SOS system in Escherichia coli decreases by about 30% upon incubation with the RecA protein, and its cofactors ATP [or its non-hydrolysable analogue adenosine-5'-O- (3-thiotriphosphate), ATP gamma S] Mg2+ and single-stranded DNA. bmh firma
LexA Protein - an overview ScienceDirect Topics
WebThe LexA repressor is stable in normal growing cells, with a half-life of nearly 1 h. E. coli contains approximately 1300 LexA molecules. Repressor self-cleavage commences … Web01. okt 2005. · DNA affinity assays demonstrated a specific interaction between the hox regulatory promoter region and a protein which, using mass spectrometry, was identified to be LexA. Overexpressed His-tagged Synechocystis LexA and EMSA showed a specific binding to the promoter region of the hox operon. WebIn this review, we discuss the structure of LexA protein, particularly with respect to distinct conformations that enable repression of SOS genes via specific DNA binding or repressor cleavage during the response to DNA damage. These may provide new starting points in the battle against the emergence of bacterial pathogens and the spread of ... bmh feedgear