WebNucAway™ Spin Columns P/N AM10070 Guaranteed RNase- and DNase-free, Ambion NucAway Spin Columns pro- vide a fast, efficient way to remove unincorporated … WebThe total T7 transcripts (unpurified ppp-ssRNA1) were digested with DNase I and then chromatographed on NucAway Spin columns (Ambion) to remove unincorporated nucleotides and DNA fragments. Slightly radiolabeled ([ -32P]CTP or ([ -32P]UTP, 0.37 MBq) T7 transcripts were further electro- phoresed on 10% preparative denaturing gels.
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