WebApr 1, 2024 · T4 DNA ligase: New England Biolabs: Cat#M0202L: BbsI: New England Biolabs: Cat#R3539S: T4 polynucleotide kinase: New England Biolabs: Cat#M0201S: CutSmart buffer, 10 ... T4 ligase : 1 μL: Total : 10 μL: Critical: 1 μL of ddH 2 O instead of sgRNA in the ligation system served as a negative control. If there are many colonies on … http://sjgl.swu.edu.cn/lab/orders/product/38070041025137
T4 DNA Ligase NEB
WebThe development of efficient methods for the detection of T4 DNA ligase is extremely important for public health. The present work demonstrates the integration of engineerable oxidase nanozyme of LaMnO 3.26 nanomaterials for the colorimetric detection of T4 DNA ligase. Specifically, the LaMnO 3.26 nanomaterials exhibited oxidase-like activity, … WebM0202LVIAL T4 DNA Ligase 10037474 Pass B0202AVIAL T4 DNA Ligase Reaction Buffer 10041766 Pass Assay Name/Specification Lot # 10042433 Non-Specific DNase Activity … portmans stores brisbane
Ligation Protocol with T4 DNA Ligase (M0202) v3 - ResearchGate
WebUsing T4 RNA ligase, a DNA (3' end) or RNA (5' end) oligonucleotide is ligated to RNA ends followed by cDNA synthesis, PCR amplification, cloning and sequencing. This method determines 5' ends, 3' polyadenylation sites and the size of poly(A) tails, and should be applicable to non-polyadenylated mRNAs and to non-message RNAs. ... WebJun 18, 2024 · T4 DNA ligase: New England Biolabs: Cat#M0202L: BbsI: New England Biolabs: Cat#R3539S: ... T4 ligase: 1 μL: Total: 10 μL: 8. Transformation. Transfer 10 μL of ligation product into 100 μL of competent Stbl3 strain, incubate the mixture on ice for 1 h, heat-shock at 42°C for 90 s and return it immediately on ice for 3 min. WebT4 DNA Ligase, NEB. Login to see prices. Brand: New England Biolabs. Characteristics of T4 DNA Ligase, NEB: Catalyzes the formation of a phosphodiester bond between juxtaposed 5′ phosphate and 3′ hydroxyl termini in duplex DNA or RNA. ... M0202L-20°C: 100,000 units: 400,000 units/ml: Additional information. Dimensions: 40 × 80 cm: portmans size chart